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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has been noticed in lots of tumors, which ends up in chromosome instability, and thus may perhaps contribute to tumorigenesis. This protein was found to associate with, and be associated with, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a essential part in advertising mobile-cycle progression within the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, happen to be explained. Two supplemental splice variants ended up documented but thorough nucleotide sequence details is not really nevertheless obtainable.
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Blocking peptides are peptides that bind specifically towards the target antibody and block antibody binding. These peptide normally is made up of the epitope acknowledged via the antibody.
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This tutorial outlines more feasible optimization of cellular and immunoassay parameters to be sure the very best effects are...
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This manual outlines more achievable optimization of cellular and immunoassay parameters to make certain the absolute best benefits are...
Antibodies certain to the blocking peptide no more bind on the epitope about the focus on protein. By comparing the staining from your blocked antibody compared to the antibody alone, you can see which staining is unique.
Concentrate on Facts Cyclin E belongs to your hugely conserved cyclin household, whose associates are characterized by a extraordinary periodicity in protein abundance in the mobile cycle. Cyclins operate as regulators of CDK kinases. Distinct cyclins exhibit distinctive expression and degradation designs which contribute into the temporal coordination of every mitotic occasion. Cyclin E forms a posh with and features as a regulatory subunit of CDK2, whose exercise is required for mobile cycle G1/S transition. This protein accumulates in the G1-S section boundary and is also degraded as cells progress by S stage.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both equally antibodies...
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